integrated development environment (version 1.0.143) Search Results


r-studio 1.0.143  (RStudio)
90
RStudio r-studio 1.0.143
R Studio 1.0.143, supplied by RStudio, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/integrated+development+environment+%28version+1%2E0%2E143%29/pmc06158314-143-2-2?v=RStudio
Average 90 stars, based on 1 article reviews
r-studio 1.0.143 - by Bioz Stars, 2026-08
90/100 stars
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rstudio© software  (RStudio)
90
RStudio rstudio© software
Rstudio© Software, supplied by RStudio, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/integrated+development+environment+%28version+1%2E0%2E143%29/pmc09549366-89-6-5?v=RStudio
Average 90 stars, based on 1 article reviews
rstudio© software - by Bioz Stars, 2026-08
90/100 stars
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software version 1.0.143  (RStudio)
90
RStudio software version 1.0.143
Software Version 1.0.143, supplied by RStudio, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/integrated+development+environment+%28version+1%2E0%2E143%29/pmc06627889-72-17-21?v=RStudio
Average 90 stars, based on 1 article reviews
software version 1.0.143 - by Bioz Stars, 2026-08
90/100 stars
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for linux version 1.0.143  (RStudio)
90
RStudio for linux version 1.0.143
For Linux Version 1.0.143, supplied by RStudio, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/integrated+development+environment+%28version+1%2E0%2E143%29/pmc07399898-90-21-26?v=RStudio
Average 90 stars, based on 1 article reviews
for linux version 1.0.143 - by Bioz Stars, 2026-08
90/100 stars
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v vulnificus  (DSMZ)
92
DSMZ v vulnificus
Normalization and quantification using Vibrio- Sequins. (A) Scatter plot showing the linearity of expected copy number concentrations (cp/µl) vs. measured copy number concentrations (cp/µl) of Vibrio- Sequins in individual DNA libraries (S28-S47). Expected copies refer to the copy numbers calculated for each standard after spiking to the samples. These numbers are based on the dPCR-quantified stock solutions of the Vibrio- Sequins. Measured copy numbers refer to the dPCR-measured copy numbers after samples have undergone library preparation. Shown are copy numbers (cp/µl) of individual replicates for each of the six standards HC1 (plume), LC1 (dark blue), rplA (petrol), ushA (green), valS (light green) and xni (yellow) for both Vibrio- Sequin mixes (1 = circles and 2 = squares) as well as the means (black) ± coefficients of variation (%CV; n = 10). (B) Quantitative accuracy of Vibrio- Sequin sequencing within individual DNA libraries (S28-S47). Scatter plots show the unnormalized coverage of individual replicates for each of the six standards HC1 (lila), LC1 (violet), rplA (pink), ushA (red), valS (orange) and xni (yellow) for both Vibrio- Sequin mixes (1 = circles and 2 = squares) against the input concentration of the Vibrio- Sequins (attomoles/µl). (C) Quantitative accuracy of Vibrio- Sequin sequencing within individual DNA libraries (S28-S47). Scatter plots show the normalized coverage of individual replicates for each of the six standards HC1 (lila), LC1 (violet), rplA (pink), ushA (red), valS (orange) and xni (yellow) for both Vibrio- Sequin mixes (1 = circles and 2 = squares) against the input concentration of the Vibrio- Sequins (attomoles/µl). Normalization occurred through subsampling reads to the lowest coverage of Vibrio- Sequins within a DNA library. (D) Three different Vibrio mixtures (A, B and C) were assembled from Qubit-quantified DNA, comprising different amounts of DNA from the Vibrio species V. cholerae, V. parahaemolyticus, V. <t>vulnificus</t> and V. metschnikovii and spiked with either Vibrio -Sequin mix 1 (Mixtures A and B) or Vibrio- Sequin mix 2 (Mixture C) at 2% fractional abundance. (E) Quantification of Vibrio -derived DNA using Vibrio- Sequins in the three Vibrio Mixtures (A, B and C) employing the method developed in . dPCR-quantified copy number concentrations (cp/µl) of individual Vibrio- Sequins within the DNA libraries (S28, S29 and S38 = Vibrio Mixtures A-C) were used for the quantification
V Vulnificus, supplied by DSMZ, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/integrated+development+environment+%28version+1%2E0%2E143%29/pmc10318669-277-16-18?v=DSMZ
Average 92 stars, based on 1 article reviews
v vulnificus - by Bioz Stars, 2026-08
92/100 stars
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ck 14 antibody  (Proteintech)
97
Proteintech ck 14 antibody
Normalization and quantification using Vibrio- Sequins. (A) Scatter plot showing the linearity of expected copy number concentrations (cp/µl) vs. measured copy number concentrations (cp/µl) of Vibrio- Sequins in individual DNA libraries (S28-S47). Expected copies refer to the copy numbers calculated for each standard after spiking to the samples. These numbers are based on the dPCR-quantified stock solutions of the Vibrio- Sequins. Measured copy numbers refer to the dPCR-measured copy numbers after samples have undergone library preparation. Shown are copy numbers (cp/µl) of individual replicates for each of the six standards HC1 (plume), LC1 (dark blue), rplA (petrol), ushA (green), valS (light green) and xni (yellow) for both Vibrio- Sequin mixes (1 = circles and 2 = squares) as well as the means (black) ± coefficients of variation (%CV; n = 10). (B) Quantitative accuracy of Vibrio- Sequin sequencing within individual DNA libraries (S28-S47). Scatter plots show the unnormalized coverage of individual replicates for each of the six standards HC1 (lila), LC1 (violet), rplA (pink), ushA (red), valS (orange) and xni (yellow) for both Vibrio- Sequin mixes (1 = circles and 2 = squares) against the input concentration of the Vibrio- Sequins (attomoles/µl). (C) Quantitative accuracy of Vibrio- Sequin sequencing within individual DNA libraries (S28-S47). Scatter plots show the normalized coverage of individual replicates for each of the six standards HC1 (lila), LC1 (violet), rplA (pink), ushA (red), valS (orange) and xni (yellow) for both Vibrio- Sequin mixes (1 = circles and 2 = squares) against the input concentration of the Vibrio- Sequins (attomoles/µl). Normalization occurred through subsampling reads to the lowest coverage of Vibrio- Sequins within a DNA library. (D) Three different Vibrio mixtures (A, B and C) were assembled from Qubit-quantified DNA, comprising different amounts of DNA from the Vibrio species V. cholerae, V. parahaemolyticus, V. <t>vulnificus</t> and V. metschnikovii and spiked with either Vibrio -Sequin mix 1 (Mixtures A and B) or Vibrio- Sequin mix 2 (Mixture C) at 2% fractional abundance. (E) Quantification of Vibrio -derived DNA using Vibrio- Sequins in the three Vibrio Mixtures (A, B and C) employing the method developed in . dPCR-quantified copy number concentrations (cp/µl) of individual Vibrio- Sequins within the DNA libraries (S28, S29 and S38 = Vibrio Mixtures A-C) were used for the quantification
Ck 14 Antibody, supplied by Proteintech, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/integrated+development+environment+%28version+1%2E0%2E143%29/pmc11862566-90-2-16?v=Proteintech
Average 97 stars, based on 1 article reviews
ck 14 antibody - by Bioz Stars, 2026-08
97/100 stars
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hmpref0833 10143  (ATCC)
93
ATCC hmpref0833 10143
Normalization and quantification using Vibrio- Sequins. (A) Scatter plot showing the linearity of expected copy number concentrations (cp/µl) vs. measured copy number concentrations (cp/µl) of Vibrio- Sequins in individual DNA libraries (S28-S47). Expected copies refer to the copy numbers calculated for each standard after spiking to the samples. These numbers are based on the dPCR-quantified stock solutions of the Vibrio- Sequins. Measured copy numbers refer to the dPCR-measured copy numbers after samples have undergone library preparation. Shown are copy numbers (cp/µl) of individual replicates for each of the six standards HC1 (plume), LC1 (dark blue), rplA (petrol), ushA (green), valS (light green) and xni (yellow) for both Vibrio- Sequin mixes (1 = circles and 2 = squares) as well as the means (black) ± coefficients of variation (%CV; n = 10). (B) Quantitative accuracy of Vibrio- Sequin sequencing within individual DNA libraries (S28-S47). Scatter plots show the unnormalized coverage of individual replicates for each of the six standards HC1 (lila), LC1 (violet), rplA (pink), ushA (red), valS (orange) and xni (yellow) for both Vibrio- Sequin mixes (1 = circles and 2 = squares) against the input concentration of the Vibrio- Sequins (attomoles/µl). (C) Quantitative accuracy of Vibrio- Sequin sequencing within individual DNA libraries (S28-S47). Scatter plots show the normalized coverage of individual replicates for each of the six standards HC1 (lila), LC1 (violet), rplA (pink), ushA (red), valS (orange) and xni (yellow) for both Vibrio- Sequin mixes (1 = circles and 2 = squares) against the input concentration of the Vibrio- Sequins (attomoles/µl). Normalization occurred through subsampling reads to the lowest coverage of Vibrio- Sequins within a DNA library. (D) Three different Vibrio mixtures (A, B and C) were assembled from Qubit-quantified DNA, comprising different amounts of DNA from the Vibrio species V. cholerae, V. parahaemolyticus, V. <t>vulnificus</t> and V. metschnikovii and spiked with either Vibrio -Sequin mix 1 (Mixtures A and B) or Vibrio- Sequin mix 2 (Mixture C) at 2% fractional abundance. (E) Quantification of Vibrio -derived DNA using Vibrio- Sequins in the three Vibrio Mixtures (A, B and C) employing the method developed in . dPCR-quantified copy number concentrations (cp/µl) of individual Vibrio- Sequins within the DNA libraries (S28, S29 and S38 = Vibrio Mixtures A-C) were used for the quantification
Hmpref0833 10143, supplied by ATCC, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/integrated+development+environment+%28version+1%2E0%2E143%29/pmc08600607-7-0-13?v=ATCC
Average 93 stars, based on 1 article reviews
hmpref0833 10143 - by Bioz Stars, 2026-08
93/100 stars
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bovine serum  (GE Healthcare)
95
GE Healthcare bovine serum
Normalization and quantification using Vibrio- Sequins. (A) Scatter plot showing the linearity of expected copy number concentrations (cp/µl) vs. measured copy number concentrations (cp/µl) of Vibrio- Sequins in individual DNA libraries (S28-S47). Expected copies refer to the copy numbers calculated for each standard after spiking to the samples. These numbers are based on the dPCR-quantified stock solutions of the Vibrio- Sequins. Measured copy numbers refer to the dPCR-measured copy numbers after samples have undergone library preparation. Shown are copy numbers (cp/µl) of individual replicates for each of the six standards HC1 (plume), LC1 (dark blue), rplA (petrol), ushA (green), valS (light green) and xni (yellow) for both Vibrio- Sequin mixes (1 = circles and 2 = squares) as well as the means (black) ± coefficients of variation (%CV; n = 10). (B) Quantitative accuracy of Vibrio- Sequin sequencing within individual DNA libraries (S28-S47). Scatter plots show the unnormalized coverage of individual replicates for each of the six standards HC1 (lila), LC1 (violet), rplA (pink), ushA (red), valS (orange) and xni (yellow) for both Vibrio- Sequin mixes (1 = circles and 2 = squares) against the input concentration of the Vibrio- Sequins (attomoles/µl). (C) Quantitative accuracy of Vibrio- Sequin sequencing within individual DNA libraries (S28-S47). Scatter plots show the normalized coverage of individual replicates for each of the six standards HC1 (lila), LC1 (violet), rplA (pink), ushA (red), valS (orange) and xni (yellow) for both Vibrio- Sequin mixes (1 = circles and 2 = squares) against the input concentration of the Vibrio- Sequins (attomoles/µl). Normalization occurred through subsampling reads to the lowest coverage of Vibrio- Sequins within a DNA library. (D) Three different Vibrio mixtures (A, B and C) were assembled from Qubit-quantified DNA, comprising different amounts of DNA from the Vibrio species V. cholerae, V. parahaemolyticus, V. <t>vulnificus</t> and V. metschnikovii and spiked with either Vibrio -Sequin mix 1 (Mixtures A and B) or Vibrio- Sequin mix 2 (Mixture C) at 2% fractional abundance. (E) Quantification of Vibrio -derived DNA using Vibrio- Sequins in the three Vibrio Mixtures (A, B and C) employing the method developed in . dPCR-quantified copy number concentrations (cp/µl) of individual Vibrio- Sequins within the DNA libraries (S28, S29 and S38 = Vibrio Mixtures A-C) were used for the quantification
Bovine Serum, supplied by GE Healthcare, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/integrated+development+environment+%28version+1%2E0%2E143%29/10__1128_slash_jvi__02202___15-47-23-26?v=GE+Healthcare
Average 95 stars, based on 1 article reviews
bovine serum - by Bioz Stars, 2026-08
95/100 stars
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2× easy taq supermix  (TransGen biotech co)
90
TransGen biotech co 2× easy taq supermix
Normalization and quantification using Vibrio- Sequins. (A) Scatter plot showing the linearity of expected copy number concentrations (cp/µl) vs. measured copy number concentrations (cp/µl) of Vibrio- Sequins in individual DNA libraries (S28-S47). Expected copies refer to the copy numbers calculated for each standard after spiking to the samples. These numbers are based on the dPCR-quantified stock solutions of the Vibrio- Sequins. Measured copy numbers refer to the dPCR-measured copy numbers after samples have undergone library preparation. Shown are copy numbers (cp/µl) of individual replicates for each of the six standards HC1 (plume), LC1 (dark blue), rplA (petrol), ushA (green), valS (light green) and xni (yellow) for both Vibrio- Sequin mixes (1 = circles and 2 = squares) as well as the means (black) ± coefficients of variation (%CV; n = 10). (B) Quantitative accuracy of Vibrio- Sequin sequencing within individual DNA libraries (S28-S47). Scatter plots show the unnormalized coverage of individual replicates for each of the six standards HC1 (lila), LC1 (violet), rplA (pink), ushA (red), valS (orange) and xni (yellow) for both Vibrio- Sequin mixes (1 = circles and 2 = squares) against the input concentration of the Vibrio- Sequins (attomoles/µl). (C) Quantitative accuracy of Vibrio- Sequin sequencing within individual DNA libraries (S28-S47). Scatter plots show the normalized coverage of individual replicates for each of the six standards HC1 (lila), LC1 (violet), rplA (pink), ushA (red), valS (orange) and xni (yellow) for both Vibrio- Sequin mixes (1 = circles and 2 = squares) against the input concentration of the Vibrio- Sequins (attomoles/µl). Normalization occurred through subsampling reads to the lowest coverage of Vibrio- Sequins within a DNA library. (D) Three different Vibrio mixtures (A, B and C) were assembled from Qubit-quantified DNA, comprising different amounts of DNA from the Vibrio species V. cholerae, V. parahaemolyticus, V. <t>vulnificus</t> and V. metschnikovii and spiked with either Vibrio -Sequin mix 1 (Mixtures A and B) or Vibrio- Sequin mix 2 (Mixture C) at 2% fractional abundance. (E) Quantification of Vibrio -derived DNA using Vibrio- Sequins in the three Vibrio Mixtures (A, B and C) employing the method developed in . dPCR-quantified copy number concentrations (cp/µl) of individual Vibrio- Sequins within the DNA libraries (S28, S29 and S38 = Vibrio Mixtures A-C) were used for the quantification
2× Easy Taq Supermix, supplied by TransGen biotech co, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/integrated+development+environment+%28version+1%2E0%2E143%29/ppr0394606-65-16-19?v=TransGen+biotech+co
Average 90 stars, based on 1 article reviews
2× easy taq supermix - by Bioz Stars, 2026-08
90/100 stars
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q-q plot  (RStudio)
90
RStudio q-q plot
Normalization and quantification using Vibrio- Sequins. (A) Scatter plot showing the linearity of expected copy number concentrations (cp/µl) vs. measured copy number concentrations (cp/µl) of Vibrio- Sequins in individual DNA libraries (S28-S47). Expected copies refer to the copy numbers calculated for each standard after spiking to the samples. These numbers are based on the dPCR-quantified stock solutions of the Vibrio- Sequins. Measured copy numbers refer to the dPCR-measured copy numbers after samples have undergone library preparation. Shown are copy numbers (cp/µl) of individual replicates for each of the six standards HC1 (plume), LC1 (dark blue), rplA (petrol), ushA (green), valS (light green) and xni (yellow) for both Vibrio- Sequin mixes (1 = circles and 2 = squares) as well as the means (black) ± coefficients of variation (%CV; n = 10). (B) Quantitative accuracy of Vibrio- Sequin sequencing within individual DNA libraries (S28-S47). Scatter plots show the unnormalized coverage of individual replicates for each of the six standards HC1 (lila), LC1 (violet), rplA (pink), ushA (red), valS (orange) and xni (yellow) for both Vibrio- Sequin mixes (1 = circles and 2 = squares) against the input concentration of the Vibrio- Sequins (attomoles/µl). (C) Quantitative accuracy of Vibrio- Sequin sequencing within individual DNA libraries (S28-S47). Scatter plots show the normalized coverage of individual replicates for each of the six standards HC1 (lila), LC1 (violet), rplA (pink), ushA (red), valS (orange) and xni (yellow) for both Vibrio- Sequin mixes (1 = circles and 2 = squares) against the input concentration of the Vibrio- Sequins (attomoles/µl). Normalization occurred through subsampling reads to the lowest coverage of Vibrio- Sequins within a DNA library. (D) Three different Vibrio mixtures (A, B and C) were assembled from Qubit-quantified DNA, comprising different amounts of DNA from the Vibrio species V. cholerae, V. parahaemolyticus, V. <t>vulnificus</t> and V. metschnikovii and spiked with either Vibrio -Sequin mix 1 (Mixtures A and B) or Vibrio- Sequin mix 2 (Mixture C) at 2% fractional abundance. (E) Quantification of Vibrio -derived DNA using Vibrio- Sequins in the three Vibrio Mixtures (A, B and C) employing the method developed in . dPCR-quantified copy number concentrations (cp/µl) of individual Vibrio- Sequins within the DNA libraries (S28, S29 and S38 = Vibrio Mixtures A-C) were used for the quantification
Q Q Plot, supplied by RStudio, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/integrated+development+environment+%28version+1%2E0%2E143%29/pmc05765123-237-11-13?v=RStudio
Average 90 stars, based on 1 article reviews
q-q plot - by Bioz Stars, 2026-08
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mycobacterium smegmatis  (ATCC)
99
ATCC mycobacterium smegmatis
Normalization and quantification using Vibrio- Sequins. (A) Scatter plot showing the linearity of expected copy number concentrations (cp/µl) vs. measured copy number concentrations (cp/µl) of Vibrio- Sequins in individual DNA libraries (S28-S47). Expected copies refer to the copy numbers calculated for each standard after spiking to the samples. These numbers are based on the dPCR-quantified stock solutions of the Vibrio- Sequins. Measured copy numbers refer to the dPCR-measured copy numbers after samples have undergone library preparation. Shown are copy numbers (cp/µl) of individual replicates for each of the six standards HC1 (plume), LC1 (dark blue), rplA (petrol), ushA (green), valS (light green) and xni (yellow) for both Vibrio- Sequin mixes (1 = circles and 2 = squares) as well as the means (black) ± coefficients of variation (%CV; n = 10). (B) Quantitative accuracy of Vibrio- Sequin sequencing within individual DNA libraries (S28-S47). Scatter plots show the unnormalized coverage of individual replicates for each of the six standards HC1 (lila), LC1 (violet), rplA (pink), ushA (red), valS (orange) and xni (yellow) for both Vibrio- Sequin mixes (1 = circles and 2 = squares) against the input concentration of the Vibrio- Sequins (attomoles/µl). (C) Quantitative accuracy of Vibrio- Sequin sequencing within individual DNA libraries (S28-S47). Scatter plots show the normalized coverage of individual replicates for each of the six standards HC1 (lila), LC1 (violet), rplA (pink), ushA (red), valS (orange) and xni (yellow) for both Vibrio- Sequin mixes (1 = circles and 2 = squares) against the input concentration of the Vibrio- Sequins (attomoles/µl). Normalization occurred through subsampling reads to the lowest coverage of Vibrio- Sequins within a DNA library. (D) Three different Vibrio mixtures (A, B and C) were assembled from Qubit-quantified DNA, comprising different amounts of DNA from the Vibrio species V. cholerae, V. parahaemolyticus, V. <t>vulnificus</t> and V. metschnikovii and spiked with either Vibrio -Sequin mix 1 (Mixtures A and B) or Vibrio- Sequin mix 2 (Mixture C) at 2% fractional abundance. (E) Quantification of Vibrio -derived DNA using Vibrio- Sequins in the three Vibrio Mixtures (A, B and C) employing the method developed in . dPCR-quantified copy number concentrations (cp/µl) of individual Vibrio- Sequins within the DNA libraries (S28, S29 and S38 = Vibrio Mixtures A-C) were used for the quantification
Mycobacterium Smegmatis, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/integrated+development+environment+%28version+1%2E0%2E143%29/us11549199-273-84-86?v=ATCC
Average 99 stars, based on 1 article reviews
mycobacterium smegmatis - by Bioz Stars, 2026-08
99/100 stars
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v.1.0.143  (RStudio)
90
RStudio v.1.0.143
Normalization and quantification using Vibrio- Sequins. (A) Scatter plot showing the linearity of expected copy number concentrations (cp/µl) vs. measured copy number concentrations (cp/µl) of Vibrio- Sequins in individual DNA libraries (S28-S47). Expected copies refer to the copy numbers calculated for each standard after spiking to the samples. These numbers are based on the dPCR-quantified stock solutions of the Vibrio- Sequins. Measured copy numbers refer to the dPCR-measured copy numbers after samples have undergone library preparation. Shown are copy numbers (cp/µl) of individual replicates for each of the six standards HC1 (plume), LC1 (dark blue), rplA (petrol), ushA (green), valS (light green) and xni (yellow) for both Vibrio- Sequin mixes (1 = circles and 2 = squares) as well as the means (black) ± coefficients of variation (%CV; n = 10). (B) Quantitative accuracy of Vibrio- Sequin sequencing within individual DNA libraries (S28-S47). Scatter plots show the unnormalized coverage of individual replicates for each of the six standards HC1 (lila), LC1 (violet), rplA (pink), ushA (red), valS (orange) and xni (yellow) for both Vibrio- Sequin mixes (1 = circles and 2 = squares) against the input concentration of the Vibrio- Sequins (attomoles/µl). (C) Quantitative accuracy of Vibrio- Sequin sequencing within individual DNA libraries (S28-S47). Scatter plots show the normalized coverage of individual replicates for each of the six standards HC1 (lila), LC1 (violet), rplA (pink), ushA (red), valS (orange) and xni (yellow) for both Vibrio- Sequin mixes (1 = circles and 2 = squares) against the input concentration of the Vibrio- Sequins (attomoles/µl). Normalization occurred through subsampling reads to the lowest coverage of Vibrio- Sequins within a DNA library. (D) Three different Vibrio mixtures (A, B and C) were assembled from Qubit-quantified DNA, comprising different amounts of DNA from the Vibrio species V. cholerae, V. parahaemolyticus, V. <t>vulnificus</t> and V. metschnikovii and spiked with either Vibrio -Sequin mix 1 (Mixtures A and B) or Vibrio- Sequin mix 2 (Mixture C) at 2% fractional abundance. (E) Quantification of Vibrio -derived DNA using Vibrio- Sequins in the three Vibrio Mixtures (A, B and C) employing the method developed in . dPCR-quantified copy number concentrations (cp/µl) of individual Vibrio- Sequins within the DNA libraries (S28, S29 and S38 = Vibrio Mixtures A-C) were used for the quantification
V.1.0.143, supplied by RStudio, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/integrated+development+environment+%28version+1%2E0%2E143%29/pmc06030475-88-26-31?v=RStudio
Average 90 stars, based on 1 article reviews
v.1.0.143 - by Bioz Stars, 2026-08
90/100 stars
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Image Search Results


Normalization and quantification using Vibrio- Sequins. (A) Scatter plot showing the linearity of expected copy number concentrations (cp/µl) vs. measured copy number concentrations (cp/µl) of Vibrio- Sequins in individual DNA libraries (S28-S47). Expected copies refer to the copy numbers calculated for each standard after spiking to the samples. These numbers are based on the dPCR-quantified stock solutions of the Vibrio- Sequins. Measured copy numbers refer to the dPCR-measured copy numbers after samples have undergone library preparation. Shown are copy numbers (cp/µl) of individual replicates for each of the six standards HC1 (plume), LC1 (dark blue), rplA (petrol), ushA (green), valS (light green) and xni (yellow) for both Vibrio- Sequin mixes (1 = circles and 2 = squares) as well as the means (black) ± coefficients of variation (%CV; n = 10). (B) Quantitative accuracy of Vibrio- Sequin sequencing within individual DNA libraries (S28-S47). Scatter plots show the unnormalized coverage of individual replicates for each of the six standards HC1 (lila), LC1 (violet), rplA (pink), ushA (red), valS (orange) and xni (yellow) for both Vibrio- Sequin mixes (1 = circles and 2 = squares) against the input concentration of the Vibrio- Sequins (attomoles/µl). (C) Quantitative accuracy of Vibrio- Sequin sequencing within individual DNA libraries (S28-S47). Scatter plots show the normalized coverage of individual replicates for each of the six standards HC1 (lila), LC1 (violet), rplA (pink), ushA (red), valS (orange) and xni (yellow) for both Vibrio- Sequin mixes (1 = circles and 2 = squares) against the input concentration of the Vibrio- Sequins (attomoles/µl). Normalization occurred through subsampling reads to the lowest coverage of Vibrio- Sequins within a DNA library. (D) Three different Vibrio mixtures (A, B and C) were assembled from Qubit-quantified DNA, comprising different amounts of DNA from the Vibrio species V. cholerae, V. parahaemolyticus, V. vulnificus and V. metschnikovii and spiked with either Vibrio -Sequin mix 1 (Mixtures A and B) or Vibrio- Sequin mix 2 (Mixture C) at 2% fractional abundance. (E) Quantification of Vibrio -derived DNA using Vibrio- Sequins in the three Vibrio Mixtures (A, B and C) employing the method developed in . dPCR-quantified copy number concentrations (cp/µl) of individual Vibrio- Sequins within the DNA libraries (S28, S29 and S38 = Vibrio Mixtures A-C) were used for the quantification

Journal: BMC Genomics

Article Title: Vibrio -Sequins - dPCR-traceable DNA standards for quantitative genomics of Vibrio spp

doi: 10.1186/s12864-023-09429-8

Figure Lengend Snippet: Normalization and quantification using Vibrio- Sequins. (A) Scatter plot showing the linearity of expected copy number concentrations (cp/µl) vs. measured copy number concentrations (cp/µl) of Vibrio- Sequins in individual DNA libraries (S28-S47). Expected copies refer to the copy numbers calculated for each standard after spiking to the samples. These numbers are based on the dPCR-quantified stock solutions of the Vibrio- Sequins. Measured copy numbers refer to the dPCR-measured copy numbers after samples have undergone library preparation. Shown are copy numbers (cp/µl) of individual replicates for each of the six standards HC1 (plume), LC1 (dark blue), rplA (petrol), ushA (green), valS (light green) and xni (yellow) for both Vibrio- Sequin mixes (1 = circles and 2 = squares) as well as the means (black) ± coefficients of variation (%CV; n = 10). (B) Quantitative accuracy of Vibrio- Sequin sequencing within individual DNA libraries (S28-S47). Scatter plots show the unnormalized coverage of individual replicates for each of the six standards HC1 (lila), LC1 (violet), rplA (pink), ushA (red), valS (orange) and xni (yellow) for both Vibrio- Sequin mixes (1 = circles and 2 = squares) against the input concentration of the Vibrio- Sequins (attomoles/µl). (C) Quantitative accuracy of Vibrio- Sequin sequencing within individual DNA libraries (S28-S47). Scatter plots show the normalized coverage of individual replicates for each of the six standards HC1 (lila), LC1 (violet), rplA (pink), ushA (red), valS (orange) and xni (yellow) for both Vibrio- Sequin mixes (1 = circles and 2 = squares) against the input concentration of the Vibrio- Sequins (attomoles/µl). Normalization occurred through subsampling reads to the lowest coverage of Vibrio- Sequins within a DNA library. (D) Three different Vibrio mixtures (A, B and C) were assembled from Qubit-quantified DNA, comprising different amounts of DNA from the Vibrio species V. cholerae, V. parahaemolyticus, V. vulnificus and V. metschnikovii and spiked with either Vibrio -Sequin mix 1 (Mixtures A and B) or Vibrio- Sequin mix 2 (Mixture C) at 2% fractional abundance. (E) Quantification of Vibrio -derived DNA using Vibrio- Sequins in the three Vibrio Mixtures (A, B and C) employing the method developed in . dPCR-quantified copy number concentrations (cp/µl) of individual Vibrio- Sequins within the DNA libraries (S28, S29 and S38 = Vibrio Mixtures A-C) were used for the quantification

Article Snippet: Additionally, Vibrio -DNA was derived from the following reference strains; Vibrio parahaemolyticus (NCTC 10,885, NCTC 11,058), V. vulnificus (DSMZ 10,143) and V. cholerae (NCTC 8042, NCTC 11,348).

Techniques: Sequencing, Concentration Assay, Derivative Assay